FIG. 4.

Germination of sleB spores after decoating or in combination with cwlJ and cotE mutations. (A) Appropriate dilutions of intact (stippled) or decoated (solid) spores from strains PS832 (wild type) (+), FB111 (cwlJ), FB112 (sleB), FB113 (sleB cwlJ), and FB72 (ger-3) at an OD₆₀₀ of 1 were spotted on 2×YT agar medium for titer determination. Note that decoated FB113 spores were not tested and the FB72 spores were spotted after Ca²⁺-DPA treatment. (B) Appropriate dilutions of spores from PS3328 (cotE), FB112 (sleB), and PS3330 (sleB cotE) strains at an OD₆₀₀ of 1 were spotted on 2×YT medium for titer determination. Titers of wild-type spores at an OD₆₀₀ of 1 were 1.3 × 10⁸ CFU/ml. The values shown are averages of two experiments. The individual experimental values lay within 40% of the averages shown. ND, not determined.