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. 2023 Feb 11;12(4):585. doi: 10.3390/cells12040585

Figure 4.

Figure 4

β3-adrenergic receptors are responsible for alpha1A-adrenoceptors up-regulation in MSCs. (A) Fold-change in the percentage of MSCs responded to noradrenaline after sympathetic overdrive (SO) or sympathetic overdrive in the presence of the β1-antagonist CGP20712 (100 nM, CGP), β2-antagonist ICI118551 (50 nM, ICI), or β3-antagonist SR59230A (250 nM, SR) (n = 12–19). (B,C) Fold-change in the percentage of cells that responded to noradrenaline after sympathetic overdrive of either control ASC52telo cells (SO) or cells after ADRB2 (B) or ADRB3 (C) knockout (n = 4–7). (D,E) Confocal images of frozen sections of human subcutaneous adipose tissue. β3-adrenoceptor localized in PDGFRβ positive MSCs in vessels of subcutaneous adipose tissue of normotensive patients with obesity (D) and hypertensive (E) patients with obesity (double arrow). Cells expressing β3-adrenergic receptor (green), CD31 positive endothelial cells (red), alpha smooth muscle actin positive cells (red), and PDGFRβ positive MSCs (red). Nuclei stained with Dapi (blue). Scale bar 10 μm. * p < 0.05 calculated with Kruskal–Wallis and one way ANOVA on ranks.