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Sequence alignment of the UBC domain of different E2 enzymes produced by Jalview. Residues are colored according to the percentage of the residues in each column that agree with the sequence of UbcH5B (>80% dark blue, >60% medium dark blue, >40% light blue, ≤40% no color). The conserved phenylalanine and SPA motif at the L1 and L2 loops, respectively, are highlighted with red boxes.
