Figure 6.

Interaction of E3 Arkadia with UbcH13 E2 enzyme. (a) Diagram of the total CSPs measured at a 1:2 molar ratio (saturation point) for ¹⁵N Ark RING/¹⁴N UbcH13, *: represents disappeared residues, +: represents residues with no information. (b) Ark RING mapping (PDBid: 2KIZ) after the addition of UbcH13 (residues that disappeared during the interaction are colored red, whereas residues that exhibited ‘fast exchange’ interaction are colored coral). (c) Diagram of the total CSPs measured at a 1:2 molar ratio for ¹⁵N UbcH13/¹⁴N Ark RING. (d) UbcH13 mapping (PDBid: 1J7D) after the addition of Ark RING. (e) Diagram of the total CSPs measured at a 1:2 molar ratio for ¹⁵N UbcH13/¹⁴N Ark LONG. (f) UbcH13 mapping (PDBid: 1J7D) after the addition of Ark RING. (g) ITC data for the titration of UbcH13 into Ark RING and (h) into Ark LONG. (i) In vitro auto-ubiquitylation assays using UbcH13 in the presence and absence of Ark LONG (left) and in the presence of GST-Ark LONG or GST-Ark RING (right).