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. 2023 Feb 15;15(4):1224. doi: 10.3390/cancers15041224

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) The H&E stain of a hepatic tumor section contains an outlined tumor region composed of necrotic tumor with viable tumor region found at the interface to the healthy tissue. MALDI imaging of (B) the water-loss oxonium ion of GlcNAc, m/z 204.0865; and (C) the sodium-cationized water-loss oxonium ion, m/z 226.0691. (+) indicates the use of OGA, while (−) indicates an untreated serial section. The tissue sections were treated with PNGase F to remove all N-glycans prior to the OGA (+/−) treatment.