Figure 1.

Generation of iPSC-derived MN spheroids. (A) Summary of the small-molecule protocol developed by [16] and optimized by our group to generate iPSC-derived MNs as a 2D monolayer. Briefly, iPSCs are differentiated towards neural progenitor cells (NPCs) that are patterned to motor neuron neural progenitor cells (MNPCs), which are finally differentiated into MNs. (B) At the MNPCs stage, two control cell lines (3450 and AIW002-02) were dissociated and plated into 96 U-bottom ultra-low attachment well plates in MN induction and maturation medium to generate MN spheroids that were kept in culture until their analysis. (C) Representative bright-field pictures of MN spheroids after 14 and 28 days of differentiation of both control cell lines.