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. 2023 Feb 8;12(4):545. doi: 10.3390/cells12040545

Figure 3.

Figure 3

Transcript expression profile of iPSC-derived MN spheroids. Normalized expression levels of NANOG, OCT4, PAX6, OLIG2, HB9, ISL1, CHAT, MAP2, VSX2, SIM1, MBP, and GFAP in iPSCs, MNPCs, and iPSC-derived MN spheroids differentiated for 14 and 28 days from (A) 3450 and (B) AIW002-02 lines. Data normalized to Actβ-GAPDH expression. Bar graphs show the mean ± SEM; three MN spheroid batches per time point (14 and 28 days). Each MN spheroid batch was obtained from iPSC-derived MNPC batches at passage 3 generated through independent differentiation processes. Significance was determined for each gene using a one-way ANOVA. Next, post Dunnett’s tests using iPSCs (NANOG and OCT) or MNPCs (PAX6, OLIG2, HB9, ISL1, CHAT, MAP2, VSX2, SIM1, and MBP) as reference samples were performed. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.