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. 2023 Feb 8;12(4):545. doi: 10.3390/cells12040545

Table 6.

CUBIC reagent 1 (R1) and CUBIC reagent 2 (R2) compositions.

Reagents Supplier/Manufacturer Working Concentration Catalogue Number
R1
dH2O N/A 35% by wt N/A
Urea Sigma-Aldrich, St. Louis, MO, USA 25% by wt 15604
Quadrol (N, N, N’, N’-tetrakis (2-hydroxy-propyl) ethylenediamine) Sigma-Aldrich, St. Louis, MO, USA 25% by wt 122262
Triton X-100 Bioshop, Burlington, ON, Canada 15% by wt TRX506
R2
dH2O N/A 15% by wt N/A
Triethanolamine Thermo Fisher Scientific, Waltham, MA, USA 10% by wt T407-500
D-sucrose Thermo Fisher Scientific, Waltham, MA, USA 50% by wt BP220-1
Urea Sigma-Aldrich, St. Louis, MO, USA 25% by wt 15604