Figure 3.

Effects of blockade of Cxxc5 function on hair regrowth. (a–d) The dorsal skins of C57BL/6N 7-week-old wild-type mice were depilated and harvested at 12 or 20 days. Topical treatment with 10 μg PGD₂ or 10 mM PTD-DBM every other day was performed from 8 days to 12 or 20 days. (a) The relative hair shaft length 20 days after depilation (n = 10). (b) IHC staining for β-catenin at 12 days after depilation. (c) Quantitative calculations for fluorescence intensities of nuclear β-catenin (n = 10). (d) Immunoblotting analyses for β-catenin, Pcna, and total Erk at 12 days. (e–g) The back skins of 7-week-old Cxxc5 wild-type or knock-out mice were depilated and obtained at 12 or 20 days. Treatment of 10 μg PGD₂ was conducted from 8 days to 12 or 20 days every other day. (e) IHC analyses for β-catenin at 12 days. (f) Quantitative values for intensities of nuclear β-catenin (n = 10). (g) Immunoblotting assay for β-catenin, total Erk, and Pcna at 12 days. Scale bars = 100 µm. Dotted lines indicate hair follicles. Values are expressed as means ± SEM. Student’s t-tests: ** p < 0.005, *** p < 0.0005.