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. 2023 Feb 9;12(4):555. doi: 10.3390/cells12040555

Figure 5.

Figure 5

Effects of inhibition of both Cxxc5 and Gsk-3β functions on hair growth of the DHT-treated mice. (a–d) Vibrissa follicles were separated and cultured with 100 nM DHT or 0.5 μM KY19382 for 4 or 7 days. (a,b) The relative length of vibrissa follicles at 7 days (n = 34). (c) IHC staining for β-catenin at 4 days. (d) ALP staining for vibrissa follicles. Arrows indicate ALP-positive region. (e–j) The dorsal skins of C57BL/6N 8-week-old wild-type mice were shaved and topically treated daily with 100 μM DHT or 2 mM KY19382. The dorsal skins were harvested at 42 days. (e) Gross images of regrown hair in mice (n = 6). (f) ALP staining for dorsal skins. (g) H&E staining for mouse dorsal tissues. (h,i) Quantitative measurements of dermal thickness, the number of hair follicles, and hair cycle score using H&E images (n = 30). (j) IHC analyses for β-catenin and Ae13 using dorsal skins. Scale bars = 100 µm. Dashed lines represent hair follicles. Arrows mean ALP-positive region. Values are expressed as the means ± SEM. Student’s t-test: * p < 0.05, ** p < 0.005, *** p < 0.0005, N.S: not significant.