Figure 3.

Quantification of PC12 growth cone velocity of projection. Multivariate time series plot of discretized velocities of projection at different angles. (A–C, cell #1) and (D–F, cell #2). Green, purple, and gray correspond to positive, negative, and null velocities, respectively. Black squares indicate the angles where the laser was located and blue arrows the displacement of the spot. In both cells, positive velocities were registered for at least 10 min previous to the first round of irradiation, at the angles of the spot location (red dotted squares). (B,E) show the median values of the velocities of projection, and (C,F) the median values of the cumulative distance, in both cases at the angles where the spots were located during the irradiated (ON), non-irradiated (OFF), or previous to irradiation (PREV) periods.