TABLE 2.
Synthetic oligonucleotides used in this study
| Name | Oligonucleotide sequencea |
|---|---|
| Oligonucleotides based on etrA | |
| E1 | 5′-CATAGCCCAGCGTCTTGTG-3′ |
| E2 | 5′-ACGATTATGCCAAATGACTTGAGT-3′ |
| E3 | 5′-ATTGCGATCGATGAACGTCTCGCCGCCTTTA-3′ |
| E4 | 5′-ATTGCGATCGATCCTTGTTCGGTGATGGTGTAACTT-3′ |
| E5 | 5′-CTTTGTATCCCGTTCACCCTCAAT-3′ |
| E6 | 5′-ATCCTAGCATTACCCGCCAAGA-3′ |
| E7 | 5′-GGCTGCCCTGTGGACTGGTA-3′ |
| E8 | 5′-CGAAGCGGTTAGCAAGGTTACTGA-3′ |
| Oligonucleotide based on Kmr gene: K1 | 5′-ATTGCGATCGATTTATGCTTGTAAACC GTT-3′ |
| Oligonucleotides based on cat gene | |
| C1 | 5′-CAGACGGCATGATGAACCTGAATC-3′ |
| C2 | 5′-CCACCGTTGATATATCCCAATGGC-3′ |
| Oligonucleotides based on pCALc | |
| S1 | 5′-ATACGACTCACTATAGGGGAATTG-3′ |
| S2 | 5′-TGGCTGCTGAGACGGCTATGAAAT-3′ |
a
Underlined regions indicate the ClaI site engineered into oligonucleotides E3, E4, and K1.