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. 2023 Jan 29;45(2):1073–1085. doi: 10.3390/cimb45020071

Figure 5.

Figure 5

NEAT1v1 or SOD2 knockdown suppresses AKT activity independent of ER stress. (A) mRNA expression of ER stress target genes (BIP, CHOP, and ERO1α). HLF and HuH6 cell lines were transduced with adenoviruses expressing shNT, shNEAT1a, or shSOD2a in the presence of 0 mM (H2O; CTRL) or 2 mM TUDC for 48 h. * p < 0.05 vs. shNT; # p < 0.05 vs. CTRL (Tukey’s test; n = 3). (B) Representative Western blot images for the indicated proteins. βTUB is shown as an internal control. HLF and HuH6 cell lines were transduced with adenoviruses expressing shNT (N), shNEAT1a (Na), or shSOD2a (Sa) for 48 h. (C) Representative Western blot images for the indicated proteins. GAPDH is shown as an internal control. HuH6 cell lines were transduced with adenoviruses expressing shNT (N), shNEAT1a (Na), or shSOD2a (Sa) in the presence of 0 mM (H2O; CTRL) or 2 mM TUDC for 48 h.