FIG. 3.

Mobility shift analysis of purified CRG proteins with a Gal4 probe. Affinity purified G4-CAD, G4-KID and CRG were diluted 1:20, 1:50, and 1:100 and bound to 10 fmol of ³²P-labeled Gal4 probe in a standard EMSA. Bound and free probes were separated on a 5% polyacrylamide mobility shift gel. Densitometry was used to quantify the relative amounts of probe bound by the various protein samples. A representative experiment is shown.