FIG. 4.

CRG stimulates the formation of a large complex at the promoter in an Ag-EMSA. (A) The Ag-EMSA method uses a ³²P-end-labeled, 150-bp DNA probe that contains five Gal4 binding sites (5×G) and a TATA-containing minimal promoter. The probe is incubated with purified protein and RLNE under conditions that allow DNA binding (see Materials and Methods). (B) Ag-EMSA binding reactions included 5 fmol of probe incubated with 1, 5, or 10 fmol of purified G4-DBD or CRG protein and 3 μg of RLNE where indicated. Complexes were resolved and visualized as indicated in Materials and Methods.