Fig 6. Inhibition of the motility of A549 and AGS cancer cells by the acetone extract of F. cucullata and usnic acid in sub-lethal concentrations.

(A–C) Migration assay of A549 (A) and AGS (B) cells treated with the F. cucullata extract or usnic acid, and quantificational analysis of wound length in each group (C). (D–E) Invasion assay of A549 and AGS cells treated with the F. cucullata extract, usnic acid, or lichesterinic acid (D), and quantificational analysis of invaded cell numbers in each group (E). Representative images are shown from three independent experiments. Data represent mean ± S.E.M. (standard error of the mean), n = 3. **p<0.01; ***p<0.001; NS, no significant difference when compared to the dimethylsulfoxide-treated group in each cell lines. ^@@p<0.01; ^@@@p<0.001 when compared to the indicated group.