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Mutational analysis of IKK phosphorylation sites in p105. p105 cDNA fragments encoding amino acids 917 to 968 of p105 were cloned into pRSETA. Purified proteins (see Coomassie-stained SDS gel [CS], left panel) were used in an in vitro kinase assay with purified IKKβ (KA, right panel). Mutated amino acids in the p105 destruction box are shown in bold letters.
