Study flow diagram. Out of 541 collected samples, 273 were from patients with suspected active TB (a) and 268 from presumed non-infected individuals (b). After exclusion of samples not meeting inclusion/exclusion criteria, and exclusion of samples from multiple collections from the same donor and with inconsistent T-Track® TB results upon repeated testing, a total of 344 samples were analyzed, including 181 from patients with confirmed active TB (a) and 163 from confirmed non-infected controls (b). Abbreviations: HIV, human immunodeficiency virus; IGRA, interferon-gamma release assay; IS, immunosuppressive; MTC Mycobacterium tuberculosis complex; NTM, nontuberculous mycobacteria; QFT-Plus, QuantiFERON®-TB Gold Plus; TB, tuberculosis.