FIG. 3.

Phenotypic and growth characteristics of the three classes of transformants. Shown are the phenotypes of compatible (class 1 [C]) transformants, such as the introduction of a het-c^PA allele into C9-2 (PA) spheroplasts, incompatible class 2 (I²) transformants, such as introduction of a het-c^GR allele into C9-2 (PA) spheroplasts, and class 3 (I³) transformants, such as introduction of a het-c^OR allele into C9-2 (PA) spheroplasts. The genotypes of strains are given in Table 1. (a) Representative colony growth of the three classes of transformants. All are shown after 3 days of incubation at 30°C on solid VM (see Materials and Methods). (b) Average growth rate (centimeters/day) of three classes of transformants represented by the introduction of het-c^PA (C), het-c^GR (I²), and het-c^OR (I³) alleles into a C9-2 (PA) strain (see above). (c) HCD in the three classes of transformants represented by the introduction of het-c^PA (C), het-c^GR (I²), and het-c^OR (I³) alleles into a C9-2 (PA) strain (see above) after 1 and 3 days of vegetative growth. Evan's blue (16) was used to stain dead hyphal compartments as described in Materials and Methods. Magnification, ×61.