TABLE 2.
Oligonucleotide primers used for the construction of artificial het-c alleles
| Primer name | Sequencea |
|---|---|
| Red | GGA GAC ATG GCG ATA TCG |
| SP1.3 | GCT CAT GCC AGG AAC AAC |
| del3.3 | AGC GTA CTT GAG TTT GAG TTT AGT CGC AGT GC |
| del3.5 | ACT CAA ACT CAA GTA CGC TTG GCC CCT GGT |
| po1.3 | CTG AAC TGT CCA TTG TTA CGT GTG TCA |
| po1.5 | CAC GTA ACA ATG GAC AGT TCA GGA GCG TAA GA |
| po2.3 | GTC TAT TCT CAC GTG TGT CAT TCT TGA G |
| po2.5 | GAC ACA CGT GAG AAT AGA CAG TTC AGG CGC GTA AGA |
| po3.3 | CTG TCT ATT CTC GAG TGT GAT TCG CGT ATC |
| po3.5 | CAC ACT CGA GAA TAG ACA GTT CAG GCG CGT AAG A |
| po4.3 | GTG TCA TTC TTG AGT TTG AGT TTA GTC GC |
| po4.5 | CTC AAA CTC AAG AAT GAC ACA CGT AAC |
| p26m-3 | CGC CCC AGA ATC ATC TCT TTC CTC ATA TGG |
| p26m-5 | GAA AGA GAT GAT TCT GGG GCG AAG TAC ACG |
| pd1.3 | AGC GTA CTT TCC TTC TCC GGG TCT TAC |
| pd1.5 | GGA GAA GGA AAG TAC GCT TGG CCC CTG |
| pd2.3 | AGC ATA CTT TAC GCG CCT GAA CTG TCT |
| pd2.5 | AGG CGC GTA AAG TAT GCT TGG CCC CTG |
| pd3.3 | AAG CGT ACT TCG GTC TTA CGC GCC TGA A |
| pd3.5 | GTA AGA CCG AAG TAC GCT TGG CCC CTG |
| pd4.3 | AGC GTA CTT TCT ATT CTC GAG TTT GAG |
| pd4.5 | CTC GAG AAT AGA AAG TAC GCT TGG CCC CTG |
| od-3 | TCC ATT GTT GAG TGT GAT TCG AGT ATC TG |
| od-5 | ATC ACA CTC AAC AAT GGA AAG TCG GTT |
| go-3 | CTT ACG TGT GTC ATT CTT GAG TGT GAT TCG AGT AGC |
| go-5 | AAG AAT GAC ACA CGT AAG TTG GTT TGG CCC TTG G |
a
The primer sequences are shown in the 5′-to-3′ orientation.