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. 2023 Jan 21;14(2):278. doi: 10.3390/genes14020278

Figure 2.

Figure 2

Analysis of the conserved ARF DNA-binding domains, gene structure, and conserved motifs depending on the phylogenetic relationships in ARF genes. A: we built the phylogenetic tree using 35 ARF proteins with the ML method. The phylogenetic tree contains three subgroups, Class I, Class II, Class III (Class IIIa, Class IIIb), shown in different colors. B: Exon/intron structure analysis of the ARF genes. Black lines, red boxes, and green boxes indicate introns, exons, and untranslated regions (UTRs), respectively. C: Conserved motifs of the ARF genes elucidated by Multiple Expectation maximizations for Motif Elicitation (MEME). The conserved motifs are represented by the different colored boxes. D: we examined the conserved ARF domains using Pfam and the Simple Modular Architecture Research Tool (SMART). Brown boxes indicate conserved ARF DNA-binding domains, blue boxes indicate conserved ARF domains, and gray boxes indicate conserved AUX/IAA domains. The scale bar of each PyuARFs is shown at the bottom of the Figure.