Table 1. A summary of the binding affinities to TAPBPR orthologs and the peptide exchange results for 13 MHC-I representatives.
| Supertype | A01 | A01 A03 | A01 A24 | A02 | A03 | A24 | B08 | B44 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Allele | HLA-A* | HLA-B* | HLA-E* | HLA-G* | ||||||||||
| 01:01 | 30:01 | 29:02 | 02:01 | 68:02 | 03:01 | 11:01 | 24:02 | 08:01 | 37:01 | 01:03 | 01:01 | MR1 | ||
| Global population frequency (%) | 13.2 | 2.0 | 2.6 | 24.1 | 1.3 | 12.3 | 6.3 | 9.6 | 8.4 | 1.3 | 36.1 | 74.0 | ||
| hTAPBPR KD* (μM) | Loaded | NB† | 0.95 | 0.19 | 9.44 | 0.22 | n.d.‡ | NB† | 10.9 | NB† | NB† | NB† | NB† | 98.7 |
| Empty | NB† | 0.99 | 0.26 | 0.17 | 0.12 | n.d.‡ | 2.04 | 2.54 | NB† | 7.02 | 1.51 | NB† | ||
| chTAPBPR KD* (μM) | Loaded | NB† | NB† | 0.17 | NB† | 0.56 | n.d.‡ | NB† | 17.4 | 3.19 | NB† | NB† | NB† | 19.2 |
| Empty | 1.46 | 1.28 | 0.29 | 2.96 | 0.43 | n.d.‡ | 0.91 | 1.68 | 1.89 | 2.19 | NB† | 11.22 | ||
| Peptide exchange§ | hTAPBPR | No | Yes | Yes | Yes | Yes | Yes (12) | Yes | Yes | No | Yes | Yes (44) | ||
| chTAPBPR | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | ||||
*KD measured by SPR.
†NB, no binding.
‡n.d., KD not determined by SPR.
§On the basis of FP and/or DSF assays.