. 2023 Jan 30;14(2):360. doi: 10.3390/genes14020360

Table 1.

Primers and PCR conditions used in the current study.

Organisms/Genes	Sequence (5′-3′)	Amplicons Size	Annealing Temperatures	References
Ticks/cox	HCO2198: TAAACTTCAGGGTGACCAAAAAATCA	710 bp	49 °C	[23]
Ticks/cox	LCO1490: GGTCAACAAATCATAAAGATATTGG	710 bp	49 °C	[23]
Ticks/16S rDNA	16S+1: CCGGTCTGAACTCAGATCAAG T	460 bp	54 °C	[24]
Ticks/16S rDNA	16S-1: GCTCAATGATTTTTTAAATTGCTGT	460 bp	54 °C	[24]
Rickettsia/gltA	CS-78: GCAAGTATCGGTGAGGATGTAAT	401 bp	56 °C	[25]
Rickettsia/gltA	CS-323: GCTTCCTAAAATTCAATAAATCAGGAT	401 bp	56 °C	[25]
Rickettsia/ompA	Rr190.70: ATGGCGAATATTTCTCCAAAA	532 bp	55 °C	[26]
Rickettsia/ompA	Rr190.701: GTTCCGTTAATGGCAGCATCT	532 bp	55 °C	[26]
Rickettsia/ompB	120-M59: CCGCAGGGTTGGTAACTGC	862 bp	50 °C	[27]
Rickettsia/ompB	120-807: CCTTTTAGATTACCGCCTAA	862 bp	50 °C	[27]