Table 2.
Potential EV miRNA biomarkers for ALS.
| Biofluid | EV Isolation Method | Analysis | ALS Patients (n) | Controls (n) |
miRNA Identified | Results in ALS Patients Compared to Controls | Author, Year |
|---|---|---|---|---|---|---|---|
| Serum | ExoQuick/ ExoEasy Kit | qRT-PCR | 10 | 20 HC | miR-27a-3p | 1 reduced | Xu et al. (2018) [136] |
| Plasma | Polyethylene-glycol/ L1CAM immuno-precipitation | Microarray | 5 | 5 HC | miR-4736, miR-4700-5p, miR-1207-5p, miR-4739, miR-4505, miR-24-3p, miR-149-3p, miR-4484, miR-4688, miR-4298, miR-939-5p, miR-371a-5p, miR-3619-3p | 13 elevated | Katsu et al. (2019) [129] |
| miR-1268a, miR-2861, miR-4508, miR-4507, miR-3176, miR-4745-5p, miR-3911, miR-3605-5p, miR-150-3p, miR-3940-3p, miR-4646-5p, miR-4687-5p, miR-4788,miR-4674, miR-1913, miR-634, miR-3177-3p | 17 reduced | ||||||
| Plasma | Nv96 peptide | NGS | 14 | 12 HC | miR-532-3p, miR-144-3p, miR-15a-5p, miR-363-3p, miR-183-5p | 5 elevated | Saucier et al. (2019) [139] |
| miR-4454, miR-9-1-5p, miR-9-2-5p, miR-9-3-5p, miR-338-3p, miR-100-5p, miR7977, miR-1246, miR-664a-5p, miR-7641-1 miR-1290, miR-4286, miR-181a-1-5p, miR-181a-2-5p, miR-181b-1-5p, miR-181b-2-5p, miR1260b, miR-199a-1-3p, miR-199b-3p, miR-199a-2-3p, miR-127-3p, let-7c-5p | 22 reduced | ||||||
| Serum | SPB ExoQuickl/L1CAM immuno-precipitation | NGS | 20 | 20 | miR-146a-5p, miR-199a-3p, miR-151a-3p, miR-151a-5p, miR-199a-5p | 5 elevated | Banack et al. (2020) [137] |
| miR-4454, miR-10b-5p, miR-29b-3p | 3 reduced | ||||||
| CSF | Total Exosome Isolation Reagent-mediated precipitation | qRT-PCR | 14 | 9 DC 9 HC |
miR-124-3p | 1 elevated | Yelick et al. (2020) [122] |
| Serum | ExoChip | Nano String |
14 | 8 | miR-520f-3p, miR-4454, miR-7975, miR-450a-2-3p, miR-1268b, miR-26a-5p, miR-1255a, miR-342-3p, miR-127-3p, miR-551b-3p, miR-1262 | 11 elevated | Lo et al. (2021) [117] |
| miR-877-5p, miR-298, miR-766-3p, miR-587, miR-1254 | 5 reduced | ||||||
| Plasma | Centrifugation/Ultra-centrifugation | NGS | 6 | 6 HC 6 AD 9 PD 9 FTD |
miRNA-8089, miRNA-196a-5p, miRNA-3152-3p*, miRNA-607, miRNA-3607-3p, miRNA-6825-3p, miRNA-7106-5p, miRNA-3976, miRNA-4492, miRNA-200a-3p, miRNA-205-5p, miRNA-6858-3p, miRNA-1273c, miRNA-6888-3p, miRNA-4302, miRNA-4634, miRNA-182-3p, miRNA-3160-3p, miRNA-1-3p, miRNA-200a-5p, miRNA-7704, miRNA-210-3p, miRNA-31-5p, miRNA-133a-3p*, miRNA-34c-5p, miRNA-455-5p, miRNA-6842-5p, miRNA-3619-3p, miRNA-4279, miRNA-4508, miRNA-1469, miRNA-141-3p, miRNA-542-3p, miRNA-615-3p, miRNA-200c-3p, miRNA-4451*, miRNA-18a-5p, miRNA-200b-3p, miRNA-184, miRNA-9-5p, miRNA-7c-5p, miRNA-6746-5p, miRNA-3195, miRNA-206, miRNA-6068 | 45 elevated | Sproviero et al. (2021) [140] |
| miRNA-493-3p, 409-3p, miRNA-323b-3p*, miRNA-6073, miRNA-432-5p, miRNA-134-5p, miRNA-330-3p, miRNA-625-3p, miRNA-4446-3p, miRNA-148b-3p, miRNA-370-3p, miRNA-584-5p, miRNA-224-5p, miRNA-381-3p, miRNA-199a-5p, miRNA-654-3p, miRNA-335-3p, miRNA-543*, miRNA-4433b-5p*, miRNA-130b-5p, miRNA-4286, miRNA-382-5p | 22 reduced | ||||||
| Plasma | SPB ExoQuickl/L1CAM immuno-precipitation | qRT-PCR | 50 | 50 | miR-151a-5p, miR-146a-5p | 2 elevated | Banack et al. (2022) [4] |
| miR-4454, miR-10b-5p, miR-29b-3p | 3 reduced |