FIG. 2.
Promoter association of TAF130 derivatives in vivo. (A) Expression levels of the indicated (HA)3-tagged TAF130 derivatives expressed from the natural TAF130 promoter on centromeric plasmids as assayed by Western blotting using antibodies to the HA epitope or to TAF130. (B) TFIID complex formation by the indicated TAF130 derivatives. (HA)3-tagged TAF130 derivatives were immunoprecipitated, and components of the TFIID complex were detected with the indicated antibodies. (C) Cross-linked chromatin preparations from strains carrying the indicated (HA)3-tagged TAF130 derivatives or vector alone (the left panel represents the input sample) were immunoprecipitated with monoclonal antibodies against the HA tag (central panel) or polyclonal antibodies against TBP (right panel). PCR products corresponding to the indicated promoters or the POL1 structural gene are shown.