FIG. 3.
The TAF130-Δ16 derivative is transcriptionally inactive. (A) RNA levels of the indicated genes in strains containing a copper-inducible TAF130-depletion allele and plasmids expressing (HA)3-tagged wild-type TAF130 or TAF130-Δ16 at 0, 2, and 4 h after the addition of copper. The TRP3, RPL9A, and RPS8A genes are dependent on TAF130 function, whereas the PGK and tRNAw genes are not (32, 45). (B) Levels of wild-type and mutant TAF130 proteins at the indicated times after addition of copper, as determined by Western blotting with antibodies to the HA epitope or to TAF130. The position of the ubiquitin-tagged wild-type TAF130 (UbWT), which is degraded upon copper addition is also indicated.