FIG. 5.
Interaction of TFIID with activation domains. Whole cell extracts containing (HA)3-tagged wild-type TAF130 or TAF130-Δ16 were incubated with glutathione-Sepharose beads bound to GST protein (G) or to GST fusions to a mutated form of the VP16 activation domain (Δ), the GST-VP16 activation domain (V), or the TADIV activation domain of Adr1 (T). The indicated TFIID components in the bound (left panel) or supernatant (right panel) fractions were detected by Western blotting with the indicated antibodies.