Figure 3.

Pathogen leaf disc assay for dual-gene CRISPR-edited tomato leaves infected with Fol 1322 strain. (A,B) WT and dual-gene (DXS) CRISPR/Cas9 positive transformants infected with Fol 1322 strain. (C) Graphical representation of the average mean area of infected lesions of control and DXS in transient agro-infiltrated leaves. The area of lesions (mm²) was measured by Image J software and the average size of the lesion was taken for statistical significance. Bars represent the average means ± SE of measurements from 5 lesion spots from 5 different leaf discs of control (WT) and transformed dual-gene (DXS) CRISPR/Cas9 constructs. The asterisk denotes a significant difference determined by t-test (* p < 0.05, ** p < 0.01, *** p < 0.001). PID: post-infection day. WT: wild-type.