FIG. 2.

Effect of infection on viability of wortmannin-treated scid (S33) cells. (A) Viability of scid cells after infection with the IN⁺ virus. Cells were infected at an MOI of 4 in the absence of wortmannin (□), in the presence of 1 μM wortmannin (▵), or in the presence of 2 μM wortmannin (). As a control, cells were mock infected in the absence of wortmannin (●), in the presence of 1 μM wortmannin (▴), or in the presence of 2 μM wortmannin (⧫). Cells were harvested at the indicated times, and viability was measured by trypan blue dye exclusion. An average of two independent counts is shown. (B) Viability of wortmannin-treated S33 cells after infection with the integrase-defective, IN⁻ virus. Cells were infected with the IN⁻ virus at an MOI of 4 in the absence of wortmannin (●), in the presence of 1 μM wortmannin (▴), or in the presence of 2 μM wortmannin (⧫). In addition, cells were infected with the IN⁺ virus (controls and symbols as in Fig. 2A). Viability was again measured by trypan blue dye exclusion; two independent counts were made at each time point.