Table 2.
Predicted and experimental values of the response variables at optimal conditions for SCO2 and other extraction methods.
| Characteristics | Extraction Methods | |||
|---|---|---|---|---|
| SCO2-aqEtOH | UA-EtOH 2 | PH-H2O 3 | ||
| Predicted | Experimental 1 | Experimental | ||
| Y (%, w/w) | 22.024 | 24.05 ± 1.54 B | 23.81 ± 0.92 B | 27.90 ± 1.60 A |
| TPC (mg GAE/g) | 77.109 | 76.58 ± 4.25 AB | 81.02 ± 3.07 A | 68.90 ± 3.94 B |
| CC (mg/g) | 4.453 | 4.65 ± 0.39 A | 4.36 ± 0.86 A | 3.90 ± 0.21 A |
| TAC (Cy3GE/g) | 10.598 | 10.89 ± 1.56 A | 6.05 ± 0.75 B | 5.28 ± 0.41 B |
| Cy3GC (mg/g) | 9.023 | 9.05 ± 1.02 A | 4.39 ± 0.42 B | 4.08 ± 0.51 B |
| TAADPPH (mmol TE/g) | 190.976 | 193.2 ± 7.4 B | 206.1 ± 11.3 AB | 228.3 ± 16.3 A |
| TAACUPRAC (mmol TE/g) | 789.168 | 798.2 ± 12.8 B | 840.3 ± 17.5 B | 930.5 ± 24.4 A |
Different letters indicate significant differences between the extracts (p < 0.05). 1 Extract obtained at optimum conditions of SCO2 extraction, 90:10 (CO2: aqueous EtOH), solvent-solid ratio 1:25, 60 min (dynamic). Desirability = 0.995; 2 Extract obtained by ultrasound assisted ethanol at 24 °C, solvent-solid ratio 1:10, 60 min (static); 3 Extract obtained by pressurized hot water at 75 °C, 100 bar, solvent-solid ratio 1:10, 60 min (static); Abbreviations: Y, yield; TPC, total phenolic content; CC, catechin content; TAC, total anthocyanin content; Cy3GC, cyanidin-3-O-glucoside content; TAA-DPPH and TAA-CUPRAC, total antioxidant activity by DPPH and CUPRAC assays. All in dry basis.