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. 2023 Jan 26;14(2):319. doi: 10.3390/genes14020319

Figure 2.

Figure 2

goa-1[R209H] and goa-1[E246K] animals exhibit aberrant motor behavior. (A) Trajectories of 15 worms on agar plates recorded for 2 minutes. Different colors refer to different animals. The gene-edited mutants showed abnormal crawling and longer (goa-1[R209H]) or irregular (goa-1[E246K]) tracks compared with the controls. (B) goa-1[R209H] crawled faster than wild-type animals (*** p < 0.0001; one-way ANOVA with Bonferroni correction). The aberrant motor behavior of both mutants was also revealed by the number of body bends per minute (C), the time spent in a coiled position (D), and the reversal rate (E) (* p < 0.05 and *** p < 0.0001). Twenty animals were tested for each genotype. Data represent means ± SEM of multiple observations. Data collected in our previous study [18] are included in panels B–E for comparison (gray bars). sa734 is a null allele of goa-1 [30,32].