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Quantity of circular targeting construct and circular CRISPR construct.
| 10 µg + 10 µg | 5 µg + 5 µg | 2.5 µg + 2.5 µg | 1 µg + 1 µg | |
| Project 1: Positive clones by long-range PCR (%) | 67/93 (72%) | 79/93 (84.9%) | 41/48 (85.4%) | 35/40 (87.5%) |
| Project 2: Positive clones by long-range PCR (%) | 18/93 (19.4%) | ND | ND | 5/93 (5.4) |
