FIG. 6.
Cdk2 activity regulation in hypoxic cells. (A) Cdk2 activity in hypoxia-treated cells, measured by immune-complex kinase assay. Autoradiograms of phosphorylated Histone H1 were scanned and then analyzed by using ImageQuant, and 32P incorporation was plotted as a percentage of the control. (B) Histone H1 autoradiogram and Western blots of Cdk2, cyclin E, and cyclin A from a representative time course. (C) Kinase activity and Cdk2 content of cyclin A and cyclin E immunoprecipitates from control and hypoxic cell extracts.