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. 2023 Feb 17;14(2):512. doi: 10.3390/genes14020512

Figure 6.

Figure 6

RT-qPCR validation of differentially expressed transcripts. Transcripts selected for the RT-PCR validation of RNA-seq were mybpc1, septin10, strada, ppp3cc, myom2, tsc1, robo2, insr, rab9a, fos, and bach2. (a) Validation between vehicle and DOC; (b) validation between DOC and DOC plus mifepristone; (c) validation between DOC and DOC plus eplerenone. For RNA-seq, in purple, “#” indicates a log2 fold change ≥2.0 and FDR <0.05. For RT-qPCR, in blue, relative expression was normalized against fau and actβ, and “*” indicates significant differences in fold change from vehicle or DOC groups (mean ± SEM, n = 4, p < 0.05). Abbreviations: mybpc1, myosin-binding protein C1; strada, STE20-related kinase adapter protein α; ppp3cc, calcineurin; myom2, myomesin 2; tsc1, TSC complex subunit 1a; robo2, roundabout homolog 2; insr, insulin receptor; rab9a, ras-related protein Rab-9A; fos, proto-oncogene c-Fos; bach2, BTB domain and CNC homolog 2; fau, 40S ribosomal protein S30; actβ, β actin.