FIG. 2.

LD and DD tim and per mRNA profiles of tim promoter-tim transgenic lines. The mRNA levels for per, tim, tim⁰, and rp49 were measured in tim promoter-tim transgenic lines and Canton S flies by RPA in LD (12-h light; 12-h dark) and DD (constant darkness) conditions. “tim” denotes the transgene mRNA, “per” and “tim⁰” are endogenous per and tim⁰ mRNAs, and “rp49” is the normalization control. The genotypes are indicated. (A) A representative RPA of fly heads from flies sacrificed at high (ZT15) and low (ZT3) zeitgeber time points in LD cycles. (B) Quantitation of per and transgenic tim mRNAs from four LD experiments. (C) A representative RPA of fly heads from flies sacrificed at high (CT15) and low (CT3) circadian time points in the first day of DD after entraining for 3 days of 12:12 LD cycles. (D) Quantitation of per and transgenic tim mRNAs from two DD experiments.