FIG. 7.
In vivo functional analysis of tim promoter elements. Flies contained tim promoter-luciferase fusions. Each graph shows the average luciferase activity (y axis) of flies (n = 12) from a representative line for the genotype indicated. All experiments were done under LD conditions (12 h of light [white boxes]; 12 h of dark [black boxes]); zeitgeber times are denoted on the x axis. Mutations in the tim promoter are the same as those used in S2 cell assays. (A) Line containing a normal 756-bp tim promoter, designated timpFL-LUC. (B) Line containing a mutagenized TER1 box. (C) Line containing a mutagenized PERR box. (D) TER1/TER2 double mutant line. (E) TER1/PERR double mutant line.