Skip to main content
. 2023 Feb 17;2023:2828981. doi: 10.1155/2023/2828981

Figure 5.

Figure 5

hucMSC-Ex inhibits the expression of α-SMA, collagen I, collagen III, and fibronectin in colonic fibroblasts. (a) Western blot analysis of α-SMA, collagen I, collagen III, and fibronectin expression in human intestinal fibroblasts CCD18-Co after treatment with TGF-β for 48 hours. (b) Grayscale scanning of (a), ∗∗P < 0.01 and ∗∗∗P < 0.001. (c) QPCR analysis of α-SMA, COL1A1, COL3A1, and FN1 expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 48 hours, ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. (d) QRT-PCR analysis of COL1A1 expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 24 hours, ∗P < 0.05. (e) QRT-PCR analysis of COL1A1 expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 72 hours, ∗P < 0.05 and ∗∗P < 0.01; (f) IF analysis of α-SMA expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 48 hours (400x). (g) IF analysis of fibronectin expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 48 hours (400x).