Table 2.
Reagent | Volume |
---|---|
5x gDNA wiper mix | 2 μl |
10x RT mix | 2 μl |
HiScript III enzyme mix | 2 μl |
Oligo (dT)20VN | 1 μl |
Random hexamers | 1 μl |
RNase-free H2O | 12 μl |
Reagent | Volume |
---|---|
5x gDNA wiper mix | 2 μl |
10x RT mix | 2 μl |
HiScript III enzyme mix | 2 μl |
Oligo (dT)20VN | 1 μl |
Random hexamers | 1 μl |
RNase-free H2O | 12 μl |