Table 4.
Primer sequences.
| Species | Genes | Sequences | Annealing temperature |
|---|---|---|---|
| Mice | β-Actin | F: TCCTCCTGAGCGCAAGTACTCT | 58°C |
| R: GCTCAGTAACAGTCCGCCTAGAA | |||
| TGF-β | F: CCAGATCCTGTCCAAACTAAGG | 58°C | |
| R: CTCTTTAGCATAGTAGTCCGCT | |||
| α-SMA | F: GCGGTGGCTATTCCTTCGTGACTAC | 56°C | |
| R: CGTCAGGCAGTTCGTAGCTCTTC | |||
| COL1A1 | F: TGAACGTGGTGTACAAGGTC | 58°C | |
| R: CCATCTTTACCAGGAGAACCAT | |||
| COL3A1 | F: AGTCGGAGGAATGGGTGGCTATC | 58°C | |
| R: CAGGAGATCCAGGATGTCCAGAGG | |||
| FN1 | F: CTATAGGATTGGAGACACGTGG | 58°C | |
| R: CTGAAGCACTTTGTAGAGCATG | |||
|
| |||
| Human | β-Actin | F: TCCTCCTGAGCGCAAGTACTCT | 58°C |
| R: GCTCAGTAACAGTCCGCCTAGAA | |||
| α-SMA | F: TCGTGCTGGACTCTGGAGATGG | 58°C | |
| R: CCACGCTCAGTCAGGATCTTCATG | |||
| COL1A1 | F: AAAGATGGACTCAACGGTCTC | 60°C | |
| R: CATCGTGAGCCTTCTCTTGAG | |||
| COL3A1 | F: GGCTACTTCTCGCTCTGCTTCATC | 58°C | |
| R: TCTCTATCCGCATAGGACTGACCAAG | |||
| FN1 | F: AATAGATGCAACGATCAGGACA | 58°C | |
| R: GCAGGTTTCCTCGATTATCCTT | |||