Figure 3.

Detection of interaction of NRP1 and SARS-CoV-2 S protein in cells

Representative images of colocalization studies between FLAG-SARS-CoV-2-S1^493−685 and different HA-NRP1 constructs by confocal immunofluorescence microscopy in Vero-E6 cells. The cells were transiently transfected with FLAG-SARS-CoV-2-S1^493−685 and different mutants of HA-NRP1 as indicated. 30 h post-transfection, cells were fixed and mounted, and protein expression patterns were visualized using a Leica SP8 White Light Laser Confocal System. Scale bars represent 10 μm. The images shown are representative from three independent biological experiments (average 100 cells were observed per experimental condition per replicate).