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. 2023 Feb 25;26(4):106274. doi: 10.1016/j.isci.2023.106274

Figure 5.

Figure 5

Reduced expression of SARS-CoV-2 S protein is related to NRP1 functional mutation

(A) 293T cells were transfected with the indicated HA-NRP1 (wild type and 4Cys-4Ala mutant) and FLAG-SARS-CoV-2-S1493−685 constructs at different ratio. Cells were lysed 30 h post transfection, and the protein expression was analyzed.

(B) Quantification of the band intensities (n = 3). FLAG-SARS-CoV-2-S1493−685 band intensities were normalized to the actin band and further normalized to FLAG-SARS-CoV-2-S1493−685 level in presence of NRP1-WT for each experimental set up. Mean and SD are indicated. Data were analyzed by one-way ANOVA: ∗,p < 0.05; ∗∗,p < 0.005; ∗∗∗, p < 0.0005.

(C) 293T cells were transfected with the indicated HA-NRP1 (wild type and 4Cys-4Ala mutant) and FLAG-SARS-CoV-2-S constructs at 1:1 ratio. Cells were lysed 30 h post transfection, and the protein expression was analyzed.

(D and E) Immunoblot analysis showing altered expression of exogenously expressed SARS-CoV-2-S1493−685 or (e) SARS-CoV-2-S in Vero-E6 cells in presence of NRP1-4Cys-4Ala mutant.