Figure 7.

Reduced cellular expression of pERK was observed in cells co-transfected with SARS-CoV-2 S protein and NRP1 mutant

(A) Vero-E6 cells were transfected with the indicated HA-NRP1 constructs together with FLAG-SARS-CoV-2-S1^493−685. Cells were lysed, and endogenous ERK phosphorylation was analyzed.

(B) Quantification of the band intensities (n = 3). pERK band intensities were normalized to the Actin control. Data are represented as mean ± SD, and ∗∗, p < 0.005 (Two-tailed Student’s t test).

(C) Immunoblot analysis of whole-cell lysate derived from Vero-E6 cells transfected with the indicated constructs. Upregulation of ERK phosphorylation was abrogated in cells co-transfected with SARS-CoV-2 S protein and NRP1 mutant.