FIG. 4.

Interaction between NF-Y and ATF6α(A) or ATF6β (B) detected by two-hybrid assays. Full-length NF-YA was fused with GAL4BD in pBIND to express GAL4BD-NF-YA fusion protein, whereas unfused full-length NF-YB or NF-YC was expressed from a pcDNA3.1(+)-based plasmid. Various subregions of ATF6α and ATF6β were fused with VP16AD in pACT to express various VP16AD fusion proteins, as indicated. HeLa cells were transiently transfected with a combination of bait plasmid (total, 100 ng) and prey plasmid (100 ng) together with reporter plasmid pG5luc (100 ng). Relative luciferase activity was determined and is presented as in Fig. 2.