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. 2023 Jan 3;42(9):665–678. doi: 10.1038/s41388-022-02588-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Spatiotemporal view of development from a single cell to a multicellular organoid. Gray: cell membrane, LCK-GFP. Magenta: nuclei, H2B-mCherry. A The development of a type 1 organoid is dominated by canonical mitosis without forming any prominent fecundity cell structure. The sphere exhibits typical glandular features with relatively uniform cells. The original single cell is labeled o1. White arrows: original cell and its progeny cells; cyan arrow: cell-cell boundary. B The development of a type 2 organoid involves generating fecundity cells and forming complex tissue structures with pleomorphic nuclear morphology. The original single cell is labeled o2. The roman letters i and ii denote the daughter cells. The suffixes -a and -b indicate their generation; the suffixes -a* and -b* indicate the two daughter cells from the second round of mitosis. c–f White arrows: host PGCC, magenta arrows: fecundity cells. g, h White arrows: host PGCC; Magenta arrows: fecundity cell at metaphase and its daughter cells.; yellow arrows: a new fecundity cell (cell i-b*) generated from mitosis of cell-i. j White arrow: giant chromosome assembly at metaphase in the host PGCC; magenta arrow: released fecundity cell. k White arrows: bipolar mitotic figure from a daughter cell. The time format is hours: minutes: seconds. Bars equal 20 μm.