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. 2023 Jan 3;42(9):665–678. doi: 10.1038/s41388-022-02588-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A The time-series images demonstrate giant cell cycle configurations that underlie MEM. a-ab. Time-series images of FUCCI-labeled MDA-HGSC-2414 cells over approximately 148 h. G1 phase marker: CDT1-mKO2, red; S/G2/M phase marker: GEMININ-mAG, green; G1/S transition period: yellow. NEB nuclear envelope breakdown, NER nuclear envelope recovery, G1 G1 phase, G1/S Trans G1/S transition, G2 G2 phase, M metaphase. ac A line chart shows the dynamic fluctuations of the G1/G2-dependent fluorescence intensity (FI). The time format is hours: minutes: seconds. Bar equals 20 μm. B Time-lapse images of a PGCC underwent RMEM. a–n Time-lapse images of FUCCI-labeled MDA-HGSC-2414 cells over approximately 148 h. Phase contrast images are shown below the corresponding fluorescence images. The experimental procedures and color-rendering methods are consistent with those in panel A. o Same as A, panel ac. Bars equal 20 μm.