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. 2023 Jan 3;42(9):665–678. doi: 10.1038/s41388-022-02588-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Cell proliferation kinetics of a type 1 organoid with minimal PGCCs. a, b (left panels) spindles (α-tubulin-GFP, green) and nuclei (H2B-mCherry, magenta). a, b (right panels) Corresponding color-coded images for H2B-mCherry fluorescence, indicating relative DNA contents. Insets i show typical mitotic figures of canonical mitosis. Inset ii shows tripolar open mitosis (cyan arrows). The time format is hours: minutes: seconds. Bar equals 100 μm. c–e Quantitative analyses from the time-lapse images. c Dynamic change in average nuclear diameter (left y-axis) and absolute nuclear number (right y-axis). The data represent the mean ± S.D. for the average nuclear diameter values. d Distribution of the mitotic event incidence (the numbers of all observed mitotic events, including bipolar and multipolar types, on the analyzed image) during the expansion of the cell colony. e Percentages of diploid and polyploid nuclei during organoid growth. B Cell proliferation kinetics of a type 2 organoid with abundant PGCCs. The composition, layout, and measurements are consistent with A. Inset iii series represents a tripolar mitotic event; inset iv series represent a multipolar mitotic event. The suffix -a indicates the H2B-mCherry fluorescence (grayscale), and the suffix -b indicates the α-tubulin-GFP fluorescence (green).