FIG. 5.

(A) Plasmid constructs containing the bcl2 promoter region P1 linked to the cat reporter gene were transfected into Rat1, Rat1-Myc, and A94 cell lines. Acetylation of ¹⁴C-chloramphenicol, which reflects activity of the bcl2 promoter, was quantified. Data are expressed as fold induction of the promoter activity relative to that of the vector control, which harbored the cat gene but lacked the bcl2 promoter. (B) bcl2-cat promoter-reporter plasmids were introduced into HeLa cells along with increasing concentrations of a gadd153 expression vector, and bcl2 promoter activity was determined. Data are reported as fold induction relative to the activity of the bcl2 promoter in HeLa cells transfected with a vector lacking the gadd153 gene (CMVneo). (C) bcl2-cat promoter-reporter plasmids were introduced in HeLa cells along with 1 μg of expression vector for CMVneo, wild-type Gadd153, Gadd153 harboring mutations in the leucine zipper domain (L134A/L141A), or Gadd153 harboring mutations in the transactivation domain (S79A/S82A). bcl2 promoter activity was determined 48 h later. Data are expressed relative to the activity of the bcl2 promoter-transfected empty CMVneo vector. All data are averages of at least three independent experiments and bars represent standard errors of the means.