Extended Data Fig. 8. Amiloride-sensitive sodium conductance in two-dimensional culture.

a, Data tables from voltmeter experiments using hESC-derived collecting duct cells. The transepithelial resistances and voltages were directly measured, from which short circuit currents were calculated and normalized to surface area of the transwell filter. b-c, Transepithelial voltage and resistance measurements in response to varying concentrations, including both pre- and post-amiloride treatment. Data shown (n=4 per dose) are representative of at least three independent experiments. d, Quantification of RNA-seq expression levels of potassium channels from microdissected mouse CD segments⁴¹, including connecting tubule (CNT), cortical CD (CCD), outer medullary CD (OMCD) and inner medullary CD (IMCD). Kcnj1 and Kcnj10 were absent in the IMCD, while Kcnj13 and Kcnj16 were expressed in all CD regions. n=7 independent biological replicates per segment. e, In day 18 CD organoids, only KCNJ16 was expressed at high levels in the scRNA-seq dataset. Column and error bars represent mean and standard deviation, respectively.