(A). Schematic representation of PCO procedure and open field recording. (B). Representative images illustrating heat maps of voluntary movements of sham- and PCO-treated wild type C57BL/6J mice. (C-E). PCO procedure markedly reduced total moving distance (C), and total time spent moving (D), but increased total time spent stationary (E). ***P<0.001, ****P<0.0001, two-way ANOVA, n=6 mice per group. (F). Representative images illustrating heat maps of voluntary movements in Piezo2fl/fl (left) and Trpv1Cre::Piezo2fl/fl (right) mice after PCO surgery in open field test. (G-I). Trpv1Cre::Piezo2fl/fl mice showed increased total moving distance (G) and time spent moving (H) and decreased time spent stationary (I) compared with Piezo2fl/fl mice. *P<0.05, two-way ANOVA, n=6 mice per group. All data are expressed as mean ± S.E.