Figure 3. Overview of a microphysiological system (MPS) also referred to as “Organ-on-Chip”.
(A) Bright-field image of the MPS network shows vascular channels are connected to the tissue compartment via 3 μm pores. (B) A MPS chip on the microscope stage, showing the outline of the vascular channels and tubing connected to inlet and outlet ports which allow introduction of various fluids and cells into MPS. (C) Fluorescence images show human lung microvascular endothelial cells grown to confluency in the vascular channel forming a complete 3D lumen. F-actin is labeled green using phalloidin and nuclei is labeled blue using Draq5. (Scale bar = 100 μm)